摘要(Abstract)：
为了得到一种高效的肠道集聚性大肠杆菌基因组脱氧核糖核酸(DNA)提取方法,比较OMEGA、天根、宝生物、全式金4种细菌基因组DNA提取试剂盒对肠道集聚性大肠杆菌基因组DNA的提取效果,并对宝生物、全式金试剂盒提取方法进行优化;测定所提取的基因组DNA的纯度及浓度,并进行基因组完整性检验及特征基因检测。结果表明,使用全式金细菌基因组DNA提取试剂盒提取肠道集聚性大肠杆菌基因组DNA时,通过增加溶菌酶孵育时长至40 min,同时蛋白酶K的用量增加1倍,基因组DNA提取浓度可提高约14.5倍。
关键词(KeyWords)： 肠道集聚性大肠杆菌;基因组脱氧核糖核酸;毒力基因;食品检测
基金项目(Foundation): 国家重点研发计划项目(2017YFC1601400);; 山东省“外专双百计划”项目(WST2017004);; 山东省农业科学院农业科技创新工程项目(CXGC2017B06,CXGC2017A01);; 山东省“泰山学者”建设工程项目(TSQN201909168)
作者(Author): 杨焕蝶,杨金玉,陈相艳,王易芬,郑琳琳,陈蕾蕾
DOI: 10.13349/j.cnki.jdxbn.20201112.001
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