摘要(Abstract)：
为了实现全程自养脱氮工艺在处理含有机物废水中的应用，考察有机物存在时全程自养脱氮工艺的启动、运行工况与微生物群落结构演变，通过低溶解氧和生物膜菌种富集的控制策略，缓解有机物对厌氧氨氧化菌的抑制，实现全程自养脱氮工艺启动和功能微生物的富集。结果表明，当进水化学需氧量质量浓度为600 mg/L时，全程自养脱氮工艺启动，在反应器稳定运行期间总无机氮去除率最高达到79.46%;三维荧光光谱显示腐殖酸类和富里酸类物质是主要的微生物代谢产物；微生物高通量测序显示厌氧氨氧化菌的优势属Candidatus Kuenenia和Candidatus Brocadia的相对丰度分别从0.02%和0.01%增加到10.47%和4.45%,成功实现厌氧氨氧化菌的富集。
关键词(KeyWords)： 全程自养脱氮工艺;有机物;微生物;三维荧光光谱;高通量测序
基金项目(Foundation):国家自然科学基金项目(21906064)
作者(Author): 翟方帅,孙雅萍,司光超,张馨文,乔壮明,魏东
DOI: 10.13349/j.cnki.jdxbn.20240005.003
参考文献(References)：
[1] CHAN-PACHECO C R,VALENZUELA E I,CERVANTES F J,et al.Novel biotechnologies for nitrogen removal and their coupling with gas emissions abatement in wastewater treatment facilities[J].Science of the Total Environment,2021,797:149228.
[2] 廖德祥，李小明，曾光明，等.全程自养脱氮新工艺[J].中国给水排水，2004(4):31.
[3] LI X L,ZHANG J,ZHANG X Y,et al.Start-up and nitrogen removal performance of CANON and SNAD processes in a pilot-scale oxidation ditch reactor[J].Process Biochemistry,2019,84:134.
[4] 王超超，张星星，王垚，等.全程自养脱氮工艺的研究与工程应用展望[J].工业水处理，2021,41(5):8.
[5] CHAMCHOI N,NITISORAVUT S,SCHMIDT J E.Inactivation of ANAMMOX communities under concurrent operation of anaerobic ammonium oxidation (ANAMMOX) and denitrification[J].Bioresource Technology,2008,99(9):3331.
[6] XIE J X,GUO M L,XIE J W,et al.COD inhibition alleviation and anammox granular sludge stability improvement by biochar addition[J].Journal of Cleaner Production,2022,345:125434.
[7] 张方斋，王淑莹，彭永臻，等.CANON工艺处理实际晚期垃圾渗滤液的启动实验[J].化工学报，2016,67(9):3910.
[8] BLACKBURNE R,YUAN Z G,KELLER J.Partial nitrification to nitrite using low dissolved oxygen concentration as the main selection factor[J].Biodegradation,2008,19(2):303.
[9] 孟倩倩.废水处理过程中溶解性微生物产物生成特性研究[D].济南：济南大学，2020:15-16.
[10] LIU Y R,WEI D,XU W Y,et al.Nitrogen removal in a combined aerobic granular sludge and solid-phase biological denitrification system:system evaluation and community structure[J].Bioresource Technology,2019,288:121504.
[11] WANG Z W,WU Z C,TANG S J.Characterization of dissolved organic matter in a submerged membrane bioreactor by using three-dimensional excitation and emission matrix fluorescence spectroscopy[J].Water Research,2009,43(6):1533.
[12] XIAO H K,PENG Y Z,ZHANG Q,et al.Pre-anaerobic treatment enhanced partial nitrification start-up coupled with anammox for advanced nitrogen removal from low C/N domestic wastewater[J].Bioresource Technology,2021,337:125434.
[13] GABARRó J,HERNáNDEZ-DEL AMO E,GICH F,et al.Nitrous oxide reduction genetic potential from the microbial community of an intermittently aerated partial nitritation SBR treating mature landfill leachate[J].Water Research,2013,47(19):7066.
[14] LI J B,YE W,WEI D,et al.System performance and microbial community succession in a partial nitrification biofilm reactor in response to salinity stress[J].Bioresource Technology,2018,270:512.
[15] JI J T,PENG Y Z,LI X Y,et al.A novel partial nitrification-synchronous anammox and endogenous partial denitrification (PN-SAEPD) process for advanced nitrogen removal from muni-cipal wastewater at ambient temperatures[J].Water Research,2020,175:115690.
[16] ZHAO B,HE Y L,HUGHES J,et al.Heterotrophic nitrogen removal by a newly isolated Acinetobacter calcoaceticus HNR[J].Bioresource Technology,2010,101(14):5194.
