2025年 04期

摘要(Abstract)：

为了探究生物脱氮体系启动过程中投加磁性颗粒的作用，构建2个生物脱氮反应器R_1、 R_2,其中反应器R_2中磁性颗粒添加量为30 g/L;第1阶段进水中含氨氮和亚硝态氮，接种反硝化污泥，第2阶段进水中含氨氮，接种硝化污泥，对比研究磁性颗粒对复合脱氮体系启动过程的影响，通过对微生物群落结构的分析探究磁性颗粒的作用机制。结果表明：2个反应器均能成功启动，反应器R_1、 R_2在第1阶段均能发生以厌氧氨氧化为主的反应，并且反应器R_2中厌氧氨氧化反应占比更大；第2阶段在低溶解氧作用下形成厌氧氨氧化、短程硝化反硝化共存的复合生物脱氮体系，反应器R_2的总氮去除率更高；与接种污泥样品相比，反应器R_1、 R_2的第1阶段厌氧氨氧化菌丰度均增大，反应器R_2中厌氧氨氧化菌Candidatus＿Kuenenia丰度提升较明显，反应器R_2在第2阶段对亚硝酸盐氧化菌和亚硝酸菌Nitrosomonas具有抑制作用，陶厄式菌Thauera和热单胞菌Thermomonas丰度提升明显。

关键词(KeyWords)：生物脱氮工艺;磁性颗粒;厌氧氨氧化;短程硝化反硝化

基金项目(Foundation):国家自然科学基金项目(52100088)

作者(Author): 玄思奇,张帅,张凤军,张栋,孙绍芳,王嘉斌

DOI: 10.13349/j.cnki.jdxbn.20240604.002

参考文献(References)：

[1] DU R,PENG Y Z,CAO S B,et al.Mechanisms and microbial structure of partial denitrification with high nitrite accumulation[J].Applied Microbiology and Biotechnology,2016,100(4):2011.

[2] TAO Y,GAO D W,FU Y,et al.Impact of reactor configuration on anammox process start-up:MBR versus SBR[J].Bioresource Technology,2012,104:73.

[3] LI Y,CUI N,XUAN K,et al.Start-up performance and process kinetics of a UASB-anammox reactor at low substrate concentration[J].Journal of Environmental Chemical Engineering,2021,9(6):106726.

[4] CHEN J J,ZHANG X N,ZHOU L,et al.Metagenomics insights into high-rate nitrogen removal from municipal wastewater by integrated nitrification,partial denitrification and Anammox at an extremely short hydraulic retention time[J].Bioresource Technology,2023,387:129606.

[5] CHI W L,YANG H,ZHANG S D,et al.Nitrogen removal performance and bacterial flora analysis of a partial nitrification-anaerobic ammonium oxidation-denitrification system treating rare earth element tailings wastewater[J].Journal of Environmental Chemical Engineering,2022,10(3):107961.

[6] CUI B,YANG Q,LIU X H,et al.The effect of dissolved oxygen concentration on long-term stability of partial nitrification process[J].Journal of Environmental Sciences,2020,90:343.

[7] DUAN H R,YE L,WANG Q L,et al.Nitrite oxidizing bacteria (NOB) contained in influent deteriorate mainstream NOB suppression by sidestream inactivation[J].Water Research,2019,162:331.

[8] HU B,WANG Y L,QUAN J N,et al.Effects of static magnetic field on the performances of anoxic/oxic sequencing batch reactor[J].Bioresource Technology,2020,309:123299.

[9] XU D,JI H M,REN H Q,et al.Inhibition effect of magnetic field on nitrous oxide emission from sequencing batch reactor treating domestic wastewater at low temperature[J].Journal of Environmental Sciences,2020,87:205.

[10] 孙绍钧，沈军，王晓宇，等.磁性滤料对生物脱氮滤池微生物群落的影响[J].济南大学学报(自然科学版),2023,37(2):172.

[11] WANG W,ZHOU S X,LI R,et al.Preparation of magnetic powdered carbon/nano-Fe3O4 composite for efficient adsorption and degradation of trichloropropyl phosphate from water[J].Journal of Hazardous Materials,2021,416:125765.

[12] LI W Y,LI X Y,ZHANG Q,et al.Recent advances of partial anammox by controlling nitrite supply in mainstream wastewater treatment through step-feed mode[J].Science of the Total Environment,2023,912:168965.

[13] HOU L G,LIU Y,FAN S,et al.Magnetic field enhanced denitrification efficiency of immobilized bacterial particles[J].Water Science and Technology,2020,81(3):622.

[14] 封威，罗波，熊亚.污水处理厂出水氨氮超标的原因及解决措施[J].工程技术研究，2016(7):22.

[15] LIU X W,XU J,HUANG J L,et al.Bacteria-supported iron scraps for the removal of nitrate from low carbon-to-nitrogen ratio wastewater[J].RSC Advances,2019,9(6):3285.

[16] RIOS-DEL TORO E E,CERVANTES F J.Coupling between anammox and autotrophic denitrification for simultaneous removal of ammonium and sulfide by enriched marine sediments[J].Biodegradation,2016,27:107.

[17] LI Y G,CHEN Z G,ZhOU S W,et al.Improving stability and nitrogen removal performance of pilot-scale autotrophic process for mature landfill leachate treatment utilizing in-situ organics[J].Bioresource Technology,2023,381:129118.

[18] RIKMANN E,ZEKKER I,TOMINGAS M,et al.Comparison of sulfate-reducing and conventional Anammox upflow anaerobic sludge blanket reactors[J].Journal of Bioscience and Bioengin-eering,2014,118(4):426.

[19] LIU B B,MAO Y J,BERGAUST L,et al.Strains in the genus Thauera exhibit remarkably different denitrification regulatory phenotypes[J].Environmental Microbiology,2013,15(10):2816.

[20] 曹涛涛，徐栋，白国梁，等.以RAS固体废弃物为碳源的改进型人工湿地对养殖尾水的脱氮效果[J].水生生物学报，2022,46(10):1475.

[21] DU R,PENG Y Z,CAO S B,et al.Mechanisms and microbial structure of partial denitrification with high nitrite accumulation[J].Applied Microbiology and Biotechnology,2020,104(7):3207.

[22] SI Z,PENG Y Z,YANG A M,et al.Rapid nitrite production via partial denitrification:pilot-scale operation and microbial community analysis[J].Environmental Science:Water Research & Technology,2018,4:80.