2025年 05期

摘要(Abstract)：

针对烟叶中重要香味前体物西柏三烯二醇(CBD)含量低、干扰多且准确测定难度较大的问题，建立中心切割二维液相色谱法高效测定烟叶中α-CBD、 β-CBD含量；该法采用中心切割去除大部分干扰成分，且样品前处理简单，仅用乙酸乙酯提取烟叶粉末中的目标物CBD,经离心分离和过滤即可上机检测。结果表明：该方法灵敏度高，重复性好，线性相关系数的平方大于0.999,回收率大于93%,日内、日间重复性小于5%, α-CBD、 β-CBD的质量浓度定量限分别为1.10、 0.80 mg/L。烟叶中CBD含量受产地、品种影响较大，受部位、施氮量影响较小；同产地、同品种的青烟叶中CBD含量显著高于烤后烟叶的，必须控制烟叶烤制过程CBD的降解。

关键词(KeyWords)：二维液相色谱;中心切割;西柏三烯二醇;烟草

基金项目(Foundation):国家自然科学基金项目(22176070);; 中国烟草总公司重大科技项目[110202201033(XJ-04),110202201017(LS-01)]

作者(Author): 陈敏,徐蓬军,张玮,李俊杰,柳彩云,王秀芳,费婷,吴达

DOI:10.13349/j.cnki.jdxbn.20250710.001

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