2026年 01期

Experimental Study on Nitrogen Removal Performance of Strain Paracoccus binzhouensis wg1

摘要(Abstract):

为了评估菌株Paracoccus binzhouensis wg1(菌株wg1)脱氮能力及应用潜力,测试菌株wg1在不同氨氮、硝态氮含量的废水中对无机氮的耐受度,评估在不同培养温度下菌株的脱氮效率,并检测脱氮过程中菌体的蛋白含量。结果表明:菌株wg1对无机氮具有极高的耐受度,最大耐受质量浓度达6 000 mg/L,在温度低至15℃的条件下氨氮、硝态氮的去除率分别为63.93%、 29.70%,具有良好的环境适应力和耐低温脱氮能力;菌体蛋白质量超过总干质量的50%,有转化为微生物饲料的潜力;在模拟废水中,菌株wg1能在24 h内同时去除氨氮、硝态氮和亚硝态氮,总氮去除率可达90.39%。

关键词(KeyWords):生物脱氮;菌株;好氧反硝化;异养硝化

基金项目(Foundation):国家自然科学基金项目(32070111);; 2024年度山东省重点扶持区域引进急需紧缺人才项目(47);; 2021年度济南市“新高校20条”项目(2021GXRC088)

作者(Author):常凡哲,刘文举,李强

DOI:10.13349/j.cnki.jdxbn.20251209.001

参考文献(References):

[1] HOWARTH R W.Human acceleration of the nitrogen cycle:drivers,consequences,and steps toward solutions[J].Water Science and Technology,2004,49(5/6):7.

[2] ERISMAN J W,GALLOWAY J N,SEITZINGER S,et al.Consequences of human modification of the global nitrogen cycle[J].Philosophical Transactions of the Royal Society of London,2013,368:20130116.

[3] 王夏童,房平,赵学敏,等.水体中氮素污染危害及其治理的研究综述[J].广东化工,2021,48(5):92.

[4] 荣楠,单保庆,林超,等.海河流域河流氮污染特征及其演变趋势[J].环境科学学报,2016,36(2):420.

[5] JIANG H,ZHANG Q Q,LIU W J,et al.Isotopic compositions reveal the driving forces of high nitrate level in an urban river:Implications for pollution control[J].Journal of Cleaner Production,2021,298:126693.

[6] 任朝斌,王龙,张千千.同位素技术示踪水环境生源要素氮的来源及循环过程研究进展[J].水生态学杂志,2024,45(4):1.

[7] 国秋艳,张秋英,舒旺,等.典型城市河流硝态氮污染来源的氮氧同位素解析[J].环境工程技术学报,2023,13(5):1820.

[8] CAMARGO J A,ALONSO ??.Ecological and toxicological effects of inorganic nitrogen pollution in aquatic ecosystems:a global assessment[J].Environment International,2006,32(6):831.

[9] GAO Y,YU G R,LUO C Y,et al.Groundwater nitrogen pollution and assessment of its health risks:a case study of a typical village in rural-urban continuum,China[J].PLOS ONE,2012,7(4):e33982.

[10] FAN A M,STEINBERG V E.Health implications of nitrate and nitrite in drinking water:an update on methemoglobinemia occurrence and reproductive and developmental toxicity[J].Regulatory Toxicology and Pharmacology,1996,23(1):35.

[11] HUANG S,FU Y L,ZHANG H M,et al.Research progress of novel bio-denitrification technology in deep wastewater treatment[J].Frontiers in Microbiology,2023,14:1284369.

[12] SUN Z Y,LYU Y K,LIU Y X,et al.Removal of nitrogen by heterotrophic nitrification-aerobic denitrification of a novel metal resistant bacterium Cupriavidus sp.S1[J].Bioresource Technology,2016,220:142.

[13] LEI Y,WANG Y Q,LIU H J,et al.A novel heterotrophic ni-trifying and aerobic denitrifying bacterium,Zobellella taiwanensis DN-7,can remove high-strength ammonium[J].Applied Microbiology and Biotechnology,2016,100(9):4219.

[14] FU W L,DUAN P F,WANG Q,et al.Transcriptomics reveals the effect of ammonia nitrogen concentration on Pseudomonas stutzeri F2 assimilation and the analysis of amtB function[J].Synthetic and Systems Biotechnology,2023,8(2):262.

[15] WANG Q L,DUAN H R,WEI W,et al.Achieving stable mainstream nitrogen removal via the nitrite pathway by sludge treatment using free ammonia[J].Environmental Science and Technology,2017,51(17):9800.

[16] HAN F,LI X,ZHANG M R,et al.Solid-phase denitrification in high salinity and low-temperature wastewater treatment[J].Bioresource Technology,2021,341:125801.

[17] XU Z S,DAI X H,CHAI X L.Effect of temperature on tertiary nitrogen removal from municipal wastewater in a PHBV/PLA-supported denitrification system[J].Environmental Science and Pollution Research,2019,26(26):26893.

[18] 董怡华,王凌潇,任涵雪,等.一株耐低温异养硝化-好氧反硝化菌的分离鉴定及其脱氮特性[J].生物技术通报,2023,39(12):238.

[19] WANG F,GONG X F,MENG D,et al.Paracoccus binzhouensis sp.nov.,isolated from activated sludge[J].Archives of Microbiology,2021,203(6):3007.

[20] 王孝平,邢树礼.考马斯亮蓝法测定蛋白含量的研究[J].天津化工,2009,23(3):40.

[21] 李雪,刘思彤,陈倩.一株好氧反硝化菌的鉴定及脱氮特性研究[J].北京大学学报(自然科学版),2018,54(6):1276.

[22] 卢培利,杨涵,丁阿强,等.碳源与氮源限制下细菌代谢调节研究进展[J].微生物学报,2023,63(3):946.

[23] WU T,YANG S S,ZHONG L,et al.Simultaneous nitrification,denitrification and phosphorus removal:what have we done so far and how do we need to do in the future?[J].Science of the Total Environment,2023,856(1):158977.