2026年 02期

摘要(Abstract)：

针对ω-转氨酶催化氮杂环手性胺生物合成信息有限及相对分散的现状，综述近年来利用ω-转氨酶生物催化合成氮杂环手性胺类医药中间体的研究进展，包括五元、六元、七元的氮杂环类手性胺化合物及其在药物合成中的应用，以及新酶资源开发、蛋白工程改造、反应体系优化、酶固定化技术开发、生物反应器设计等合成工艺开发策略；重点梳理在氮杂环手性胺合成过程中ω-转氨酶来源、合成方式、底物及产物类型、合成效率和分离纯化方法方面的异同，分析酶与底物适配性、副产物抑制效应等影响反应转化效率的因素，并对该领域存在的问题和发展前景展开讨论，指出利用人工智能辅助的蛋白质结构预测和功能设计工具对ω-转氨酶进行资源开发和工程改造，是该领域今后的研究方向。

关键词(KeyWords)：ω-转氨酶;手性胺;生物催化;氮杂环手性胺;药物合成

基金项目(Foundation): 国家自然科学基金项目(42206123)

作者(Author):周泽玮，鲍洁，冯丹丹，罗泉，吕雪峰

DOI:10.13349/j.cnki.jdxbn.20250624.001

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