2026年 04期

摘要(Abstract)：

为了开发高效、安全的纳米药物递送系统，通过硅烷偶联修饰将氨丙基三乙氧基硅烷的端基氨引入到介孔二氧化硅纳米颗粒表面制备氨基化介孔二氧化硅纳米颗粒(MSN-NH₂)，表征MSN-NH₂的微观形貌与结构，检测MSN-NH₂对黄芩素的载药性能，开展MSN-NH₂诱导单层连续端粒酶永生化人微血管内皮细胞(TIME)渗漏的免疫荧光实验和MSN-NH₂对TIME细胞毒性检测。结果表明：MSN-NH₂为粒径均匀、内部孔隙均匀分布的球形颗粒，比表面积为78.44 m²/g，比孔体积为0.56 cm³/g，最可几孔径为2.39 nm，对黄芩素载药量为165.56 mg/g，药物体外释放分为快速突释和均匀缓释2个阶段，可快速起效并长期维持稳定血药浓度；当MSN-NH₂的浓度为0~200 μmol/L时，TIME单层连续细胞模型未出现渗漏且MSN-NH2对TIME细胞无明显毒性。MSN-NH₂可通过与钙离子Ca²⁺发生静电排斥，降低其与血管内皮钙黏蛋白中Ca²⁺的结合能力，进而维持细胞间黏附连接的完整性，在纳米药物治疗过程中发挥血管保护作用。

关键词(KeyWords)：介孔二氧化硅纳米颗粒; 氨基化; 药物递送系统; 黄芩素; 细胞活力

基金项目(Foundation):国家自然科学基金项目(22004048); 山东省自然科学基金项目(ZR2020QB171)

作者(Author):张国营，崔新洁，邵宇，张晴，田春蕊，王金萍

DOI:10.13349/j.cnki.jdxbn.20260423.002

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