2026年 04期

组氨酸磷酸载体蛋白激酶调控永达尔梭菌异养代谢功能

Regulation of Heterotrophic Metabolic Functions in Clostridium ljungdahlii by Histidine-containing Phospho-carrier Protein Kinase


摘要(Abstract):

为了探究永达尔梭菌Clostridium ljungdahliihprK基因CLJU_RS10755编码的HPr激酶/磷酸酶(HprK/P)在菌株碳代谢调控中的生物学功能,完善该菌株碳代谢调控机制研究,首先通过生物信息学手段对hprK基因编码蛋白的序列特征进行分析,再利用成簇规律间隔短回文重复序列基因编辑技术构建hprK基因敲除突变株,结合菌株培养表型观测与转录组测序技术,系统探究HprK/P对永达尔梭菌碳代谢的调控作用。结果表明:永达尔梭菌HprK/P蛋白与枯草芽孢杆菌模式HprK/P蛋白序列具有高度同源性;敲除hprK基因后,菌株异养生长能力显著提升,乙醇与乙酸合成产量明显升高,证实HprK/P对永达尔梭菌异养碳代谢过程存在抑制作用。转录组分析进一步证明,HprK/P可全局性调控菌株碳中心代谢、能量代谢及脂肪酸合成等关键代谢通路。

关键词(KeyWords):微生物; 蛋白激酶; 比较转录组; 永达尔梭菌; 全局转录调控因子; 碳代谢调控

基金项目(Foundation):国家自然科学基金项目(32470063, U22A20425, 32370039)

作者(Author):高欣雨,赖铭池,万赛,刘自勇,李福利,李强,谭扬

DOI:10.13349/j.cnki.jdxbn.20260604.001

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