2026年 05期

溶藻弧菌噬菌体vB_ValgW2的生物学特性及全基因组分析

Biological characteristics and whole genome analysis of Vibrio alginolyticus Phage vB_ValgW2


摘要(Abstract):

为了筛选可用于替代抗生素、防控水产养殖中溶藻弧菌感染的噬菌体资源,以溶藻弧菌为宿主菌,从某海鲜市场污泥中分离出一株溶藻弧菌噬菌体vB_ValgW2,并对其生物学特性及基因组进行系统分析。结果表明:噬菌体vB_ValgW2具有清澈透明的噬斑,在噬菌体裂解液中的效价可达4.67×10~9 PFU/mL(PFU为噬斑形成单位),裂解谱较窄,最佳感染复数为0.1,平均每个细胞的裂解量为146.67 PFU。vB_ValgW2在pH为3~12、温度为-20~5℃的环境中保持活性,对紫外照射具有一定的抵抗力。全基因组分析显示,vB_ValgW2为环状双链脱氧核糖核酸,长度为78 266 bp,鸟嘌呤-胞嘧啶的摩尔分数为38.3%,包含108个开放阅读框及3个转运核糖核酸,未发现耐药性、毒力基因或移动元件。vB_ValgW2基因组编码多种结构和功能蛋白,包括结构蛋白、核酸代谢酶及辅助代谢蛋白;比较基因组学分析显示其与Vibrio phage BUCT194基因组相似性为96.33%;噬菌体vB_ValgW2具有高效的裂解能力和良好的环境适应性。

关键词(KeyWords):微生物学;噬菌体;全基因组分析;溶藻弧菌;南美白对虾

基金项目(Foundation):国家自然科学基金项目(32471614)

作者(Author):董兰霞,张佳,宋甜甜,何慧,苏肇函,曾祥荷,樊祥宇

DOI:10.13349/j.cnki.jdxbn.20260714.001

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