2026年 05期

摘要(Abstract)：

为了探究氨基酸在海洋真菌次级代谢产物研发中的应用潜力，通过单菌株多次级代谢产物策略，在6株海洋真菌培养基中加入不同种类氨基酸，观察真菌的生长状态，并对其次级代谢产物产率、丰富度以及抗菌活性、抗氧化活性进行分析与评价。结果表明：相比于空白组，不同氨基酸对同一真菌、相同氨基酸对不同真菌次级代谢产物及生物活性的影响均有差异，部分海洋真菌在适宜的氨基酸培养基中次级代谢产物丰富度、产量和生物活性均有提高，其中菌株Penicillium sp. UJNMF0740在极性氨基酸类培养基中次级代谢产物丰富度明显提高，产量提高了44.2%,对金黄色葡萄球菌的抑制率提高了66.5%,但也有海洋真菌在设计的氨基酸培养基中的次级代谢产物没有发生明显变化，甚至产物的丰富度、产量和生物活性降低。氨基酸诱导是提高海洋真菌次级代谢产物丰富度、产量以及生物活性的重要方法，须要对不同氨基酸的作用进行评估，以发现适合不同真菌的培养条件。

关键词(KeyWords)：海洋真菌;单菌株多次级代谢产物策略;氨基酸;次级代谢产物;生物活性

基金项目(Foundation):国家自然科学基金项目(82304336);; 山东省高等学校青年创新团队发展计划项目(2022KJ096)

作者(Author):王春莹,戚钰珊,李孜怡,张君生,刘娣,张华,杨慧茹,鲍洁

DOI:10.13349/j.cnki.jdxbn.20260724.001

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